The user guide also covers the features, specifications, and software of the reader, as well as the installation and. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M5e Microplate Reader transforms each well in a microplate to a fixed. 9. Services. 0. Find new and used lab equipment and shop a wide selection of products and vendors. With three modes of detection for full spectrum absorbance, fluorescence and luminescence, the SpectraMax® Mini reader . These applications may make use of…FlexStation® 3 Benchtop Multi-Mode Microplate Reader combines Molecular Devices’ SpectraMax® M5e Multi-Mode Microplate Reader performance with an integrated 8- or 16-channel pipettor into one. PURPOSE. 2. The FilterMax™ F3 and F5 Multi-Mode Microplate Readers offer a wide set of features at an affordable price-to-performance ratio. SpectraMax® Paradigm Plate Reader Oksana Sirenko, Cathy Olsen, Vanessa Ott and Evan F Cromwell Molecular Devices, LLC, 1311 Orleans Drive, Sunnyvale, CA 94089 There has been a trend in the past decade from biochemical assays toward cell based assays. The SpectraMax® M2 and M2e Systems from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). VIS absorbance detection. Description 1. SoftMax Pro Software. VMax . The Molecular Devices SpectraMax M5 is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. The plate is set up for reading and images are acquired according to specified parameters. Gemini EM is a microplate spectrofluorometer from Molecular Devices. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. FlexStation® 3 Multi-Mode Microplate Reader. The Back Panel Figure 1. 20-plate magazine (pair) 0200-6016. Molecular Devices: EMax Plus: SpectraMax 190: SpectraMax 340PC384: SpectraMax Plus384: VERSAmax:. Temperature Control 4°C above ambient to 45°C. The plate reader optics are tunable for absorbance,. Summary of Contents for Molecular Devices SpectraMax M2. Select the Driver tab and click Update Driver. Molecular Devices SpectraMax 384 Plus Microplate Reader. 25 to 3. The following components are located on the back panel of the SpectraMax and VersaMax readers: • Power switch:Chapter 1: Introduction The SpectraMax iD3 Multi-Mode Microplate Reader from Molecular Devices is a monochromator-based, multi-mode plate reader. The Back Panel Figure 1. ELISA Workflow Using the EMax plus Microplate Reader and MultiWash+ Microplate Washer. The instruments feature a bottom-read mode ideal for whole-cell-based assays, and may beSoftMax Pro GxP Software. Sensitivity and dynamic range are demonstrated by the high Z’ factors obtained on the SpectraMax. Page 1 SpectraMax M2/M2 user guide ® A MULTI-DETECTION MICROPLATE READER WITH TWO-MODE CUVETTE PORT. When connecting for the first time to either the SpectraMax L reader or StakMax system. Laboratory automation workflows can help. To maintain confidence in the standards, Molecular Devices recommends having validation plates recertified at one-to Molecular Devices for recertification at our ISO 17025 accredited lab are disassembled, cleaned, calibrated, and Fig 2: Enzymatic response of synthetic compounds. MAXline Microplate Readers User’s Manual iii Molecular Devices Corporation Instrument Warranty Molecular Devices Corporation warrants this product against defects in material or workmanship as follows: 1) All parts of the MAXline Microplate Reader excluding the lamp assembly are warranted for aPrimary considerations when choosing a microplate reader are throughput and flexibility. Molecular Devices SpectraMax Gemini XS Microplate Fluorometer. 8×15×8. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. With three modes of detection for UV/Vis absorbance, fluorescence and luminescence, the SpectraMax®. A preconfigured. NORTH AMERICA. D SpectraMax ® M2 SpectraMax ® M2 e Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089. MycoAlert Substrate was then added to the wells, and the plate was incubated for 10 minutes at room temperature. Molecular Devices provides the complete solution covering the instrument and software: • SpectraTest® ABS1, FL1, and LM1 Validation Plates for hardware validation of absorbance, fluorescence, and luminescence modes • IQ/OQ for all microplate readersMolecular Devices technical support. Monthly Plate Verification Check (Absorbance Validation Plate) Note: Monthly Plate Verification Check is maintained by Quality Control. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. 50-plate magazine : 0200-6187. Plate pre-read measurements were made in a Costar UV plate. It is more inert and reduces compound interference. This offer includes SpectraMax i3x, SpectraMax. Table 2 summarizes the results for the uniformity test. 1. The presence of VCAM-1 indicates a positive inflammatory response. 0 × 107) RLU 0. 5. to effectively test for endotoxins in samples. Molecular DevicesThe Marker Expression Protocol is used to identify cell areas labeled with the fluorescein-conjugated anti-VCAM antibody. Endpoint, kinetic, spectrum. absorbance plate readers. Analyst GT Microplate Reader: Luminescence height = 1 mm, max integration as 1,000,000 μsec. Supplier: Molecular Devices. Perform the following steps to install the drivers: Start > Control Panel > System. , call 1-800-635-5577. Microscopes and Microplate Instrumentation. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection reader. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplateMicroplate Spectrophotometers. This can increase throughput and efficiency, thus saving time and space in the lab. . Prior to fluorescence analysis, the lid from the OmniTray plate was removed. The Back Panel Figure 1. Calcium ions (Ca2+) play a key role in cellular homeostasis involving calcium channel and GPCR function, which plays a critical role in many disease pathologies. Select Update Driver. Catalog Number: (89212-396) Supplier: Molecular Devices. (b) Fluorescence responses of probe HADP and control compounds 2–4 (each 5 µM) towards human heparanase (HPA) (1 µg) in 40 mM NaOAc buffer (pH 5. Figure 1. Due to their ability to simultaneously measure multiple wells in a single run, fluorescence plate readers are useful for conducting various assays, such as quantitative assays, enzyme assays, and ELISAs. Here, we demonstrate that with Molecular Devices SpectraMax® microplate readers and the Quant-iT PicoGreen assay, users can reliably measure concentrations as low as 50 pg/mL of double-stranded DNA. Simultaneous dual emission detection enables read times of only 2:17 for an entire 384-well plate. SpectraMax iD3 reader with touchscreen and inserted USB flash drive. 2. Scanning options are endpoint, kinetic, spectral. The adapter is required for optimum performa nce with standard 96-well and 384-. Restart the system. Figure 10: The Plate section Template Editor, showing the series set up. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Plate section without PathCheck applied. Molecular Devices' microplate readers. Instrument settings can be saved as a protocol file. These systems are hybrids that allow you to choose between. With The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. Since cells are nearly confluent, the protocol. Image courtesy of Molecular Devices. Endpoint, dual read, kinetic, fast kinetic. Description: This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with t. Monthly Plate Verification Check (Absorbance Validation Plate) Note: Monthly Plate Verification Check is maintained by Quality Control. PathCheck sensor corrects optical pathlength & pipetting variation. Microplate Readers. A control well was set up to verify the accuracy of the standard curve. Lamp source is a Xenon flash lamp (1 Joule/flash). Shop a wide selection of Molecular Devices FLIPR TETRA System's on LabX. Products : SoftMax Pro Software, SoftMax Pro GxP Software, CellReporterXpress software, General, Screenworks, pCLAMP/AxoScope, GenePix Pro, MetaXpress software. This document is copyright protected and any reproduction of this document, in whole or any part, is strictly prohibited, except as Molecular Devices may authorize in writing. Luminescence was read every five minutes for five hours with three seconds of orbital shaking before each read. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. 9. This document is copyright protected and any reproduction of this document, in whole or any part, is strictly prohibited, except as Molecular Devices may authorize in. Supplier: Molecular Devices. Plate stacker and robot integration The Gemini EM Reader can be integrated with Molecular Devices’ StakMax® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. SoftMax Pro 7. Lift up the lid over the chamber to insert or remove a cuvette. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. We obtained three HEK-293 cell lines from Clontech, each stably transfected with a different fluorescent protein. 190. Endpoint. By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time-resolved fluorescence (TRF), FRET, and AlphaScreen. Page 1 of 8 . The temperature in the plate chamber is ambient and isothermal. Also refer to the Multimode Reader Comparison Chart Features. 635. Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run. BioTek Synergy H1 Hybrid OMRF research scientist Kumar Subramani reviewed BioTek Synergy H1, stating it is a configurable multi-mode microplate reader. The two different data import options are tailored to quickly get your data into SoftMax Pro to provide a single source of software for all your data reduction needs. 312 gain calibration failed. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. 1. The cuvette must be in place when you read the plate. Get a front row view of your cells. SpectraMax® Microplate Readers and SoftMax® Pro Software. Used. This process should take about 3 - 5 minutes. Devices PathCheck® Sensor—the only patented technology available that measures the depth (optical pathlength) ofThe plate was covered and mixed on an orbital shaker for five minutes and then placed in a SpectraMax i3x reader and mixed. Monitor reader performance on demand to ensure data quality is not compromised between PM/OQ. The plate was incubated at room temperature for 50 minutes or 2. Restart the system. The concentration of total free thiols in the protein. Endpoint. SpectraMax 340 (Serial number starting with M) SpectraMax 340PC (Serial number starting with L) SpectraMax Plus (Serial number starting with P) Tmax Also refer. For more sample throughput, both readers can be easily. Important things to consider when purchasing a multimode microplate reader. Microplate Reader Shelf. 46Click the Read button in the SoftMax Pro Software. Every step is optimized to simplify analysis and reporting to support our microplate readers. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. We would like to show you a description here but the site won’t allow us. United States Phone: 800-635-5577 408-747-1700. 001 EU/mL) was added in order to test the sensitivity of the SpectraMax i3 reader. For cryo preservation cells were scaled up in 10-layer factories (Corning Cat. Details. #0200-6263) UltraVision™ Plate 384 (Brooks Life Sciences cat. 100 μL of blank or endotoxin standard (in. Shop now. If no sound is heard during initialization, contact technical support. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Molecular Devices has demonstrated the value of a variety of ELISAs for a wide range of research approaches,. Step 2: Launch SoftMax Pro Software. 0A Sunnyvale, CA Made in USA Microplate readers read biological, chemical or physical events that occur on a microtiter plate. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection reader. 18 kg. Learn how you can utilize microplate readers to drive landmark discoveries. Learn how you can utilize microplate readers to drive landmark discoveries. While many companies make instruments to detect and measure luciferase activity, identifying the right instrument for you can be difficult because of their high degree of feature variability. Uncontrolled Copy for Training and Reference Purposes Only . Set up Identify Acquire Analyze Imaging with the SpectraMax MiniMax Imaging Cytometer mirrors the plate reading workflow on the SpectraMax i3 System. Read More. 020 OD 2. 9. It has dual scanning monochromators that allow for 1 nm increment selection over the wavelength range of 250 - 850 nm. The PyroGene assay has been optimized to be linear from 0. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. #6590-0121) SpectraMax i3x® Multi-Mode Microplate Reader (Molecular Devices cat. Powered by a new, optional high-speed EMCCD camera and the new Peak Pro 2 software module, the new FLIPR® Penta High-Throughput Cellular Screening System allows you to measure and analyze peaks of human-derived induced pluripotent stem cells (hiPSCs), differentiated into. 5: Schematic of. The FLIPRTETRA, which debuts today at. Molecular Devices Flourmeteric Imaging. degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner. 0 OD for earlier models. It consists of a SpectraMax® Microplate Reader, Peak Robotics S-LAB Plate Handler with the S-LAB software, and the AquaMax® 4000 Microplate Washer. 0) at 37 °C, fluorescence intensity was recorded on a Spectramax M5 Multimode plate reader (Molecular Devices, USA) at each timepoint. 2 GxP Software is our most secure software to help you achieve full FDA 21 CFR Part 11 compliance with streamlined workflows to ensure data integrity. The AquaMax® Microplate Washer is a fully self-contained system, configurable for both 96- and 384-well microplates. Plate stacker and robot integration The Gemini EM Reader can be integrated with Molecular Devices’ StakMax® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. SpectraMax M5. What plates do you recommend for SpectraMax Plate Readers? Luminescence : Solid white plates for top reads and white plates with clear bottoms for. With optimized reagents and the industry-leading data acquisition and analysis tool, SoftMax Pro® 7 Software, the SpectraMax® iD3. ®100 μL of each endotoxin standard was added to triplicate wells of an LAL Reagent Grade Multi-well Plate. #iD3) Methods. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. ORDER. From plate readers to high-content imagers, Molecular Devices has the instruments you need to advance your discovery. The SpectraMax M2 and M2e are versatile and reliable instruments that can perform a range of applications in. Robust, high value microplate reader designed to deliver research-grade results to any laboratory. To update the driver. Cells were thawed and propagated according to the recommended method specified by the supplier. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. SpectraDrop Micro-Volume Microplate (Molecular Devices cat. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. Enables data acquisition in endpoint, kinetic, and spectral scan modes at wavelengths across the UV-visible wavelength range, with the only temperature-independent method of pathlength correction. Molecular Devices makes. Retrieving Each. SpectraMax® Plus 384 Absorbance Plate Reader, Molecular Devices. Supplier: Molecular Devices. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). Read the microplate • Make sure the purple plate adapter (if required for your plate reader) is in the microplate reader drawer. Retrieving Each. 0) OD (500 to 1100) RFU (5. together with a Molecular Devices plate. Wait 20 seconds. 313 reference gain check fail. To maximize sensitivity of the assay, it is necessary to use optimal excitation and emission wavelengths. Products : General, FilterMax F5 reader. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. Step 4: Add detection antibody – Enzyme-conjugated detection antibody binds to a second site on the. The small footprint and touchscreen control allows for easy laboratory setup with minimal investment of time, cost, and effort. You can also contact Technical Support by email. the standard plate reader applications, protecting your initial investment. This document outlines references to 21 CFR Parts 58, 211 and 820 and EudraLex Annex 15 to assess the implementation of Molecular Devices microplate readers in regulated environments. Easy-to-use touchscreen allows you to quickly set up your experiments, use preconfigured analysis methods, and view tutorial videos. SpectraMax iD5 Multi-Mode Microplate Reader, a hybrid monochromator- and filter-based, five-mode plate reader with automatic filter identification and western blot capability. Highly customizable low volume applications Molecular Devices unique SpectraDrop™ Micro-Molecular Devices delivers a robust screening platform to address real-time kinetic cell-based assays with the FLIPR Tetra® System. Longside barcode reader: 0310-5101. The plate reader optics are tunable for absorbance, fluorescence intensity,. Molecular Devices의 Microplate Reader 및 washer와 원활하게 호환되는 강력한 벤치탑 Microplate stacker입니다. The most published microplate reader control and data analysis software. FLIPR. Weight. Turn the reader on and secure the USB cable. Molecular Devices' microplate readers. Fluorescent Imaging Plate Reader (FLIPRTM ) technology to measure Ca2+ flux in cells was an important development in the early 1990’s and has played a significant role in. Examples include ELISAs for quantitation of cytokines and protein concentration determination using the Bradford assay. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. washer including mechanical, electrical, and optical components to verify operating functions are in accordance to the Molecular Devices product operational specifications. The Gemini EM can sample a variety of formats, from 6- to 384-well microplates, in different modes, such as endpoint, spectral scan, kinetic, and well scan. Read height should be optimized for Vmax® plate reader specifications. Unlike conventional plate readers, multimode microplate readers are able to perform two or more forms of detection allowing a single piece of equipment to perform a wider range of assays. On the back, in the right hand top corner, there. SpectraMax® Absorbance Microplate Readers can run both cuvette-based and microplate reader applications on the same instrument. Optimize. #ABS Plus) 4s3 ™ Semi-Automatic Sheet Heat Sealer (Brooks Life Sciences cat. Imaging System Giles Scientific Biomic V3 Automatic Plate. Search. Molecular Devices, LLC. 00 USD. Ratios for each sample were calculated as Read B/Read A. Accurate and sensitive detection of nucleic acids and proteins are critical to many experiments. The Molecular Devices SpectraMax M5E is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. This can increase throughput and efficiency, thus saving time and space in the lab. The ability to accurately quantitate cell number in multi-well microplates enables a multitude of biological applications that study cell health or proliferation. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. 0 . These robust readers have been placed in labs from. APP Automated. Cell viability는 세포막 Integrity 또는. 7°C (gradient heating design). Figure 9. Molecular Devices offers a broad range of technologies for high-throughput screening, genomic and cellular analysis, colony selection, and microplate detection. Plate Formats: 6 to 384-well microplates. With three modes of detection for UV-Vis absorbance, fluorescence and luminescence, the SpectraMax Mini reader is user upgradeable - from 2 to 3 modes, providing researchers with a versatile. Page 1 of 8 . 1. One plate carrier adapter is provided with the instrument. 190. PURPOSE. 005 EU/mL to 5. 2. The 1/absorptivity value for double stranded DNA at 260 nm is commonly assumed. Luciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range. AXON Patch-Clamp. 306 plate air cal. 3860 N First Street San Jose,CA 95134 USA +1 408. If flexibility becomes important in a growing lab supporting a variety of projects, the decision might come down to whether. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. Optimized optical path - The reader’s unique quartz fiber optic system minimizes stray light for precise readings across a microplate. Published Dec 16, 2020 | Updated Dec 16, 2020. The adapter is required for optimum performance with standard 96-well and 384-. 1. 1. Standard features include a cuvette port,. Select the Driver tab. A plate containing two columns each of 75 µL, 100 µL, 150 µL, 200 µL, 250 µL, or 300 µL of yellow buffer solution was read at 426 nm on a SpectraMax microplate reader. Narrow bandwidths deliver increased measurement accuracy and linearity over the widest range of assays, including. Category 2 and 3 microplate readers use gradient heating as well. Register to download your free eBook today. This process should take about 3 - 5 minutes. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a. 00 USD. The SpectraMax ABS Plus reader utilizes Molecular . Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers. The FlexStation 3 reader exclusively offers fluid transfer validation using Molecular Devices patented PathCheck® Feature to quantify the integrated 8- and 16-channel pipettor head performance. Set up template in SoftMax Pro and read plate on the SpectraMax M5 microplate reader: Note: IMAP FP protocols for use with FAM- and TAMRA-labeled substrates are available in SoftMax Pro 5 in the Binding Assays protocol folder. The guide also covers the software features, the specifications, and the accessories of the systems. Sunnyvale, CA - May 17, 2011 - Today, Molecular Devices, a leading provider of instruments, software, and consumables for drug discovery and life sciences research, announced that NanoRacks, LLC has chosen the SpectraMax® M5e Multi-Mode Microplate Reader for use in NASA's International Space Station; the M5e Reader will be the first. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 1. FLIPR Tetra systems are fluorescence- and luminescence-based microplate readers with an integrated 1536-, 384- or 96-pipettor. 1700 +1 800. OPTICS Mirrored optics focus the light into the sample volume, and cutoff filters are used to. Users of Molecular Devices' SpectraMax® i3 Multi-mode reader can even add an optional. Here, the CVs for each plate were less than 3, and the difference between mean onset time for the plates run on the two readers was only 7%. With the novel filter system, assays such as hydrogen peroxide detection assays and IRantibody-binding assays can be run confidently with Molecular Devices. Molecular Devices technical support is available by phone and e-mail. 6 % of reading 4. : 8 Oct 12 Page 1 of 10 Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. 304 signal level saturation. Molecular Devices' microplate readers. Expand the Port (COM & LPT) Right click on the USB Serial Port (COMx) and select Properties. Extended linear dynamic range. . In this product video, see how the SpectraMax M5 quickly reads samples. FlexIII. The 1/absorptivity value for double stranded DNA at 260 nm is commonly assumed to be \~50 µg/mL for a 1-cm pathlength3,4. The SpectraMax® i3x Multi-Mode Detection Platform measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. The instruments feature a bottom-read mode ideal for whole-cell-based assays, and may be SoftMax Pro GxP Software. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. Table 3 shows sample cell proliferation assay results for CHO-K1 cells plated according to the scheme in Table 1 and allowed to proliferate in culture for two days. S. 1. Turn the reader off by pulling the power cord. begin reading microplates with seven mouse clicks. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of. It was then read from the bottom on a SpectraMax ® i3 Multi-Mode Microplate Reader using a preconfigured protocol in SoftMax Pro Software with the settings indicated in Table 1. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. 0077 OD 0. Spectranet customer portal. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. Step. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Timothy Bolus, MPM, MBA, Compliance Program Manager. Sale price $3,495. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Detection modalities include absorbance (UV-Vis Abs), fluorescence intensity (FI), luminescence (Lumi), and all have. #I3X), equipped with the following: HTRF Detection Cartridge (Molecular Devices cat. With optimized reagents, option to operate the. The touchscreen interface provides integrated instrument control, data display, and the ability to export results over your network for statistical data analysis. Microplate readers from Molecular Devices are designed to provide consistent performance for many years. One plate carrier adapter is provided with the instrument. Plate chamber design of the SpectraMax category 1 microplate readers. 0 OD for our latest microplate readers, SpectraMax® iD3, iD5, ABS, and ABS Plus readers, and up to 2. Shop now. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. The upgradeable SpectraMax M5 Multi-Mode Microplate Reader comes with the standard five modes of detection with the upgrade option to add HTRF at a later time. A single software solution addresses your needs, from plate set-up to image analysis. Absorbance values were read on the SpectraMax iD5 reader using a preconfigured BCA assay protocol (the SpectraMax iD3 reader is also suitable for these assays, as it has the same absorbance performance as the SpectraMax iD5 reader). , attenuation mode = out. Our extensive SpectraMax® line of user-friendly microplate readers – the industry’s most cited brand – provide great flexibility for labs conducting applications ranging from ELISAs to nucleic acid and protein quantitation, and include absorbance, fluorescence, luminescence and more ( Molecular Devices ) equipment, software, reagents, and consumables to use in the operation of such Molecular Devices equipment, software, reagents, and consumables. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 1. After centrifugation, the plate was immediately read on the M5e and i3x readers using the instrument settings listed in Table 1. The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. 4: Test tube cover. M2. . The chamber contains springs that position the cuvette in the proper alignment for a read. in steps of 0. The system features 96, 384, and 1536 simultaneous liquid transfer, multi-wavelength kinetic reading, fluorescence and luminescence detection, agile internal plate handling, and elevated instrument0112-0174C 3 Contents SafetyInformation 4 Warnings,Cautions,Notes,andTips 4 Chapter1:Introduction 7 InstrumentFeatures 8 ComputerIntegration 9 PMTTechnology 9The VICTOR® Nivo™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. EUROPE. Customize your instruments, as well as automate entire workflows to meet the specific needs of your assay, method, or. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. Detection of KASP genotyping reactions For the second test, the validation kit contained 36 known samples, including 33 DNA samples and 3 no-template controls (NTC). Increase productivity with patented microplate reader technologies. Page 1 of 8 . Our microplate readers are the industry’s most cited instruments and have empowered life science researchers to advance protein and cell biology, breaking the barriers to novel, landmark discoveries. Fill a clean plate with water. Recombinant protein expression Nucleocapsid. Lower variance: Normalize your plate reader assay data to the number of cells in each well. LMax and LMax II plate readers use photon counting mode similar to the SpectraMax L reader. Contains one microplate adapter, five 24-well low volume slides, five 64-well low volume bottom slides, and five of each cover slide (2 µL and 4 µL) 0200-6267. Flexibility to read a variety of plate formats. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Load up the plates that you need to run and walk. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. Luminescence detection typically offers greater sensitivity than absorbance or fluorescence detection. Find our technical support information along with contact details. Description Figure 1. The SpectraMax L reader is easily integrated with the optional StakMax microplate stacker from Molecular Devices for automated multiplate assays. . Molecular Devices, LLC. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. 2/24/2015 Molecular Devices FlexStation 3 Russ Desnoyer Our lab needed a way to measure Calcium flux downstream of the human.